5/16/2023 0 Comments Qiagen plasmic midikit 12143LORF5 has 723 bp and encodes 241 amino acids. However, some genes have not yet been studied, such as LORF5, which is located in the UL region of the DPV CHv genome. Therefore, clarifying the gene characteristics and functions of DPV would be informative in the prevention of DP.Ĭurrently, the properties of some DPV genes have been characterized, including UL41 ( He et al., 2018), US10 ( Zhang et al., 2017), UL54 ( Liu et al., 2015, 2016, 2017 Gao et al., 2017), UL24 ( Gao et al., 2017), UL13 ( Hu et al., 2017), US2 ( Gao et al., 2015), UL49.5 ( Lin et al., 2013, 2014), UL44 ( Sun et al., 2014), UL27 ( Wang et al., 2011), UL29 ( Cheng et al., 2012), and UL16 ( He et al., 2012). Previous studies have shown that viral genes that are involved in cell-to-cell spread and immune evasion but are non-essential for replication are preferred targets for α-herpesvirus gene-deletion vaccines, such as gE and gI ( Vannie et al., 2007 Ndjamen et al., 2014 Weiss et al., 2015 Zhang et al., 2015 Dong et al., 2017). In addition, all α-herpesviruses, including DPV, also have a “cell-to-cell” spreading mechanism by which virions pass directly through cell junctions to achieve infection of adjacent cells ( Farnsworth and Johnson, 2006), enabling escape from neutralizing antibodies ( Johnson and Huber, 2002 Mateo et al., 2015). Cell-free spread occurs when virions are released from an infected cell into its surrounding environment prior to entering a new cell. DPV is a cell-free virus with two ways to spread to uninfected cells after replication in infected cells. The genome sequence of the DPV Chinese virulent strain (DPV CHv) was obtained through genome sequencing the genome has a structure that is typical of α-herpesviruses, except for a lack of terminal repeats (TRS) at the 5′ end ( Wu et al., 2012a, b You et al., 2017). DPV belongs to the α-herpesvirus subfamily and is a double-stranded linear DNA virus ( Guo et al., 2009 Xiang et al., 2012 Wu et al., 2018). Live attenuated vaccines of DP virus (DPV) have been used to treat this disease ( Qi et al., 2009 Shen et al., 2010 Yang et al., 2010 Huang et al., 2014), and a variety of new effective vaccines have also been developed in recent years ( Lian et al., 2011 Yu et al., 2012 Sun et al., 2013), which efficiently control DP but have not completely eradicated it.Īnalysis of DPV, the pathogen of DP, may provide insights for the prevention and control of DP. Vaccines are considered the most effective means for preventing DP. The incidence and mortality of infected ducklings or unprotected adult ducks reach up to 100%, resulting in substantial economic losses for the global waterfowl industry ( Yuan et al., 2007 Qi et al., 2008 Xuefeng et al., 2008). The pathological features of DP include hemorrhagic lesions in the blood vessels, gastrointestinal mucosa, and lymphoid tissues ( Dhama et al., 2017). These findings lay the foundation for in-depth functional studies of the LORF5 gene in DPV or other avian herpesviruses.ĭuck plague (DP), also known as duck virus enteritis (DVE), is an acute, febrile, septic, and contagious disease in birds within Anseriformes (such as ducks, geese, and swans). Taken together, this study sheds light on the role of the avian herpesvirus-specific gene LORF5 in the DPV proliferation life cycle. Our data revealed that the LORF5 gene contributes to the cell-to-cell transmission of DPV but is not relevant to virus invasion, replication, assembly, and release formation. In this study, to investigate the role of this unique LORF5 gene in DPV proliferation, we generated a recombinant virus that lacks the LORF5 gene by a two-step red recombination system, which cloned the DPV Chinese virulent strain (DPV CHv) genome into a bacterial artificial chromosome (DPV CHv-BAC) the proliferation law of LORF5-deleted mutant virus on DEF cells and the effect of LORF5 gene on the life cycle stages of DPV compared with the parent strain were tested. The DPV genome contains 78 open reading frames (ORFs), among which the LORF2, LORF3, LORF4, LORF5, and SORF3 genes are unique genes of avian herpesvirus. 3Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Chinaĭuck plague virus (DPV) can cause high morbidity and mortality in many waterfowl species within the order Anseriformes. 2Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.1Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
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